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FDP (Fluorescein diphosphate, tetraammonium salt)

介绍

FDP is a colorless and nonfluorescent substrate for alkaline phosphatases. Sequential alkaline phosphatase mediated hydrolysis of its two phosphate substituents yields weakly fluorescent fluorescein monophosphate followed by strongly fluorescent fluorescein (excitation/emission ~488/515 nm).

Specifications

Label: Fluorescein
Ex/Em: 488/515 nm
Detection Method: Fluorescent
Molecular Formula: C20H26N4O11P2
Molecular Weight: 560.39
CAS Number: 217305-49-2
Product Size: 2 mg
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Phosphatase Substrate
 

订购

Buy Cat.No. Product name Ex/Em(nm) Size Price¥)
C275 FDP 488/515 2 mg 1500 

Documents

Datasheet (PDF): C275
MSDS (PDF): MSDS-C275

References:

Evaluation of fluorescent compound interference in 4 fluorescence polarization assays: 2 kinases, 1 protease, and 1 phosphatase.
Turek-Etienne TC, Small EC, Soh SC, Xin TA, Gaitonde PV, Barrabee EB, Hart RF, Bryant RW
J Biomol Screen (2003) 8:176-176
 
Fluorogenic substrates for beta-D-galactosidases and phosphatases derived from fluorescein (3,6-dihydroxyfluoran) and its monomethylether.Fluorogenic substrates for beta-D-galactosidases and phosphatases derived from fluorescein (3,6-dihydroxyfluoran) and its monomethylether.
ROTMAN B, ZDERIC JA, EDELSTEIN M,
Proc Natl Acad Sci U S A (1963) 50:1-6
 
Ionization and tautomerism of oxyxanthene dyes in aqueous butanolIonization and tautomerism of oxyxanthene dyes in aqueous butanol
Mchedlov-Petrossyan NO, Tychina ON, Berezhnaya TA, Alekseeva VI, Savvina LP
Dyes Pigment (1999) 43:33-46