OmicsLink™ Expression Clone Datasheet of EX-Z0092-M02

Clone Information

Catalog No.: EX-Z0092-M02
Accession No.: Z11898	ORF Length:  1083 bp
Vector Size:  5778 bp (backbone only, ORF insert not counted)
Description: POU class 5 homeobox 1
Vector: pEZ-M02	Antibiotic:    Ampicillin
Stable Selection Marker: Neomycin
Suggested Sequencing Primers:  Forward: 5'-CAGCCTCCGGACTCTAGC-3' Reverse: 5'-TAATACGACTCACTATAGGG-3'

Vector Information for EX-Z0092-M02

Restriction Enzyme Information for EX-Z0092-M02

Table 1. Restriction Enzymes That Do Not Cleave EX-Z0092-M02

Bpu10I	BsiWI	BstEII	BstZ17I	BtrI	EcoRI	EcoRV	FseI
MluI	PacI	PmeI	PmlI	PpuMI	SacII	SalI	SanDI
SbfI	SgfI	SgrAI	SmaI	SpeI	SrfI	SwaI	XbaI
XmaI

Note: Position 1 is indicated above by the arrow pointing to the backbone start site.

Table 2. Restriction Enzymes That Cleave EX-Z0092-M02 Once

AatI	1677	AbaI	395	Acc113I	3369	Acc65I	6762	AccEBI	2668	AccIII	5732	AceII	123	AdeI	995
AflII	1726	AhaB8I	6762	AhdI	3850	AliI	2668	Aor13HI	5732	ApaCI	2668	AsiI	2668	Asp718I	6762
AspA2I	1678	AspEI	3850	AspI	1990	AspMI	1677	AssI	3369	AsuNHI	119	AtsI	1990	AvrBII	1678
AvrII	1678	BalI	1954	BamHI	2668	BbvAIII	5732	Bce751I	2668	BclI	395	BfrI	1726	BglII	5747
BlfI	5732	BlnI	1678	BmtI	123	BnaI	2668	BoxI	6728	BpuAmI	5647	BseAI	5732	BsePI	2269
BsiMI	5732	BsiQI	395	Bsp13I	5732	Bsp4009I	2668	Bsp68I	2556	Bsp98I	2668	BspA2I	1678	BspAAIII	2668
BspEI	5732	BspMII	5732	BspOVI	3850	BspTI	1726	BspXII	395	BssHII	2269	Bst98I	1726	BstBZ153I	2269
BstEZ359I	501	BstHPI	501	BstI	2668	BstIZ316I	995	BstPAI	6728	BstPZ740I	1726	BstSNI	5421	BstT7I	395
Bsu23I	5732	CauB3I	5732	CciNI	6893	CelI	2668	CpoI	2388	CsiBI	6893	CspBI	6893	CspI	2388
DpaI	3369	DraIII	995	DriI	3850	Eam1105I	3850	Ecl136II	5647	EclHKI	3850	Eco105I	5421	Eco147I	1677
Eco255I	3369	Eco53kI	5647	EcoICRI	5647	Esp4I	1726	FbaI	395	GdiI	1677	GstI	2668	HinJCI	501
HincII	501	HindII	501	HpaI	501	Kpn2I	5732	KpnI	6766	Ksp22I	395	KspAI	501	LlaG2I	119
MabI	1445	MchAI	6893	MfeI	488	MlsI	1954	Mlu23I	2668	Mlu31I	1954	MluB2I	2556	MluNI	1954
MroI	5732	MscI	1954	Msp20I	1954	MspCI	1726	MunI	488	MxaI	5647	NcrI	5747	NheI	119
NotI	6893	NruGI	3850	NruI	2556	Nsp29132II	2668	NspMACI	5747	NspSAIV	2668	OkrAI	2668	Pae18kI	5747
Pae2kI	5747	PauI	2269	PceI	1677	Pfl8I	2668	PflFI	1990	PinBII	5732	Pme55I	1677	PshAI	6728
Psp124BI	5649	PstNHI	119	PsyI	1990	PtaI	5732	RflFII	3369	RspLKII	2668	Rsr2I	2388	RsrII	2388
SacI	5649	SarI	1677	Sbo13I	2556	ScaI	3369	SexAI	1445	SnaBI	5421	SolI	2668	SpoI	2556
Sru30DI	1677	SseBI	1677	SsrI	501	SstI	5649	SteI	1677	SthI	6762	StuI	1677	SurI	2668
TelI	1990	Tth111I	1990	Uba4009I	2668	Vha464I	1726	XcmI	5922	XmaJI	1678	ZrmI	3369

Note: Position 1 is indicated above by the arrow pointing to the backbone start site.

Table 3. Restriction Enzymes That Cleave EX-Z0092-M02 Twice

AaaI	1778	AaaI	6893	AagI	635	AagI	2655	AauI	7	AauI	5767	AccB7I	5850	AccB7I	6774
AclI	1205	AclI	3621	AcpI	5753	AcpI	5795	AcpII	5850	AcpII	6774	Afa16RI	721	Afa16RI	3481
Afa22MI	721	Afa22MI	3481	AflIII	4738	AflIII	6388	AloI	1063	AloI	6560	AlwNI	4329	AlwNI	5964
AocI	6085	AocI	6820	ApaI	6034	ApaI	6754	Asp10HI	5753	Asp10HI	5795	Asp10HII	5850	Asp10HII	6774
AsuII	5753	AsuII	5795	AxyI	6085	AxyI	6820	BanIII	635	BanIII	2655	BasI	5850	BasI	6774
BavAI	1354	BavAI	1978	BavBI	1354	BavBI	1978	BavCI	635	BavCI	2655	BavI	1354	BavI	1978
BbeI	1875	BbeI	6647	Bbr7I	3255	Bbr7I	5720	BbrI	1694	BbrI	5909	BbsI	3250	BbsI	5715
Bbv16II	3250	Bbv16II	5715	BbvAII	635	BbvAII	2655	BbvII	3250	BbvII	5715	BcgI	3346	BcgI	6228
Bci29I	635	Bci29I	2655	BciBI	635	BciBI	2655	BcmI	635	BcmI	2655	BdiI	635	BdiI	2655
Bim19I	5753	Bim19I	5795	BimI	5753	BimI	5795	Bli41I	635	Bli41I	2655	Bli86I	635	Bli86I	2655
BliAI	635	BliAI	2655	BliHKI	6085	BliHKI	6820	BliRI	635	BliRI	2655	BlpI	5907	BlpI	6375
BpiI	3250	BpiI	5715	Bpu1102I	5907	Bpu1102I	6375	Bpu14I	5753	Bpu14I	5795	BpuAI	3250	BpuAI	5715
Bsa29I	635	Bsa29I	2655	BsaBI	400	BsaBI	2667	Bsc91I	3250	Bsc91I	5715	BscI	635	BscI	2655
Bse21I	6085	Bse21I	6820	Bse8I	400	Bse8I	2667	BseCI	635	BseCI	2655	BseJI	400	BseJI	2667
BseX3I	1778	BseX3I	6893	Bsh1365I	400	Bsh1365I	2667	BsiBI	400	BsiBI	2667	BsiCI	5753	BsiCI	5795
BsiXI	635	BsiXI	2655	Bsp106I	635	Bsp106I	2655	Bsp119I	5753	Bsp119I	5795	Bsp120I	6030	Bsp120I	6750
Bsp1407I	7	Bsp1407I	5767	Bsp153AI	1354	Bsp153AI	1978	Bsp1720I	5907	Bsp1720I	6375	BspBS31I	3250	BspBS31I	5715
BspCI	721	BspCI	3481	BspDI	635	BspDI	2655	BspIS4I	3250	BspIS4I	5715	BspJII	635	BspJII	2655
BspKT8I	1694	BspKT8I	5909	BspLAII	5753	BspLAII	5795	BspLAIII	1694	BspLAIII	5909	BspLU11I	4738	BspLU11I	6388
BspM39I	1354	BspM39I	1978	BspO4I	1354	BspO4I	1978	BspOVII	635	BspOVII	2655	BspR7I	6085	BspR7I	6820
BspT104I	5753	BspT104I	5795	BspTS514I	3250	BspTS514I	5715	BspXI	635	BspXI	2655	BspZEI	635	BspZEI	2655
BsrBRI	400	BsrBRI	2667	BsrGI	7	BsrGI	5767	Bst28I	635	Bst28I	2655	BstAUI	7	BstAUI	5767
BstBI	5753	BstBI	5795	BstBS32I	3250	BstBS32I	5715	BstFI	1694	BstFI	5909	BstHZ55I	2595	BstHZ55I	6600
BstNZ169I	635	BstNZ169I	2655	BstTS5I	3250	BstTS5I	5715	BstV2I	3250	BstV2I	5715	BstXI	2595	BstXI	6600
BstZI	1778	BstZI	6893	Bsu15I	635	Bsu15I	2655	Bsu36I	6085	Bsu36I	6820	BsuTUI	635	BsuTUI	2655
CaiI	4329	CaiI	5964	CbiI	5753	CbiI	5795	CcoI	889	CcoI	2374	CelII	5907	CelII	6375
Cfr6I	1354	Cfr6I	1978	ClaI	635	ClaI	2655	Csp45I	5753	Csp45I	5795	Csp68KII	5753	Csp68KII	5795
CspCI	1763	CspCI	5457	CvnI	6085	CvnI	6820	DmaI	1354	DmaI	1978	EagBI	721	EagBI	3481
EagI	1778	EagI	6893	EclI	1354	EclI	1978	EclXI	1778	EclXI	6893	Eco52I	1778	Eco52I	6893
Eco56I	887	Eco56I	2372	Eco78I	1873	Eco78I	6645	Eco81I	6085	Eco81I	6820	EcoVIII	1694	EcoVIII	5909
EgeI	1873	EgeI	6645	EheI	1873	EheI	6645	ErhB9I	721	ErhB9I	3481	Esp1396I	5850	Esp1396I	6774
EspI	5907	EspI	6375	FauNDI	249	FauNDI	5315	FspII	5753	FspII	5795	HindIII	1694	HindIII	5909
HsuI	1694	HsuI	5909	KasI	1871	KasI	6643	LcaI	635	LcaI	2655	LlaCI	1694	LlaCI	5909
Lmu60I	6085	Lmu60I	6820	LplI	635	LplI	2655	LspI	5753	LspI	5795	MamI	400	MamI	2667
MchI	1872	MchI	6644	MlaI	5753	MlaI	5795	Mly113I	1872	Mly113I	6644	MroNI	887	MroNI	2372
MstII	6085	MstII	6820	MvrI	721	MvrI	3481	NaeI	889	NaeI	2374	NarI	1872	NarI	6644
NblI	721	NblI	3481	NdaI	1872	NdaI	6644	NdeI	249	NdeI	5315	NgoAIV	887	NgoAIV	2372
NgoMIV	887	NgoMIV	2372	NmeRI	1354	NmeRI	1978	NspV	5753	NspV	5795	NunII	1872	NunII	6644
OxaNI	6085	OxaNI	6820	Pae17kI	1354	Pae17kI	1978	PciI	4738	PciI	6388	PdiI	889	PdiI	2374
PfaAII	249	PfaAII	5315	PflBI	5850	PflBI	6774	PflMI	5850	PflMI	6774	PfoI	2811	PfoI	5924
PgaI	635	PgaI	2655	PlaII	5753	PlaII	5795	Ple19I	721	Ple19I	3481	PpaAI	5753	PpaAI	5795
PpeI	6034	PpeI	6754	Psp1406I	1205	Psp1406I	3621	PspOMI	6030	PspOMI	6750	PspXI	6237	PspXI	6885
Psu161I	721	Psu161I	3481	Pun14627II	1354	Pun14627II	1978	Pvu84II	1354	Pvu84II	1978	PvuI	721	PvuI	3481
PvuII	1354	PvuII	1978	Rme21I	635	Rme21I	2655	RshI	721	RshI	3481	SauBMKI	889	SauBMKI	2374
SauHPI	889	SauHPI	2374	SauI	6085	SauI	6820	SauLPI	889	SauLPI	2374	SauNI	889	SauNI	2374
SauSI	889	SauSI	2374	SceIII	887	SceIII	2372	SdiI	1631	SdiI	5870	SenPT16I	1778	SenPT16I	6893
SfiI	1631	SfiI	5870	SfoI	1873	SfoI	6645	SfuI	5753	SfuI	5795	Slu1777I	889	Slu1777I	2374
SpmI	635	SpmI	2655	SrlI	887	SrlI	2372	SsbI	1694	SsbI	5909	SseAI	1872	SseAI	6644
SshAI	6085	SshAI	6820	Ssp1I	5753	Ssp1I	5795	Ssp27144I	635	Ssp27144I	2655	Ssp4800I	7	Ssp4800I	5767
SspBI	7	SspBI	5767	SspCI	889	SspCI	2374	SspRFI	5753	SspRFI	5795	SviI	5753	SviI	5795
Uba153AI	1354	Uba153AI	1978	UbaM39I	1354	UbaM39I	1978	Van91I	5850	Van91I	6774	XmaIII	1778	XmaIII	6893
XorII	721	XorII	3481	ZhoI	635	ZhoI	2655

Note: Position 1 is indicated above by the arrow pointing to the backbone start site.

Table 4. Restriction Enzymes That Cleave EX-Z0092-M02 Three Times

AbrI	6237	AbrI	6606	AbrI	6885	Acc36I	1759	Acc36I	2140	Acc36I	2592	Alw44I	2681	Alw44I	3178
Alw44I	4424	Ama87I	6237	Ama87I	6606	Ama87I	6885	ApaBI	1424	ApaBI	1496	ApaBI	4922	ApaLI	2681
ApaLI	3178	ApaLI	4424	AquI	6237	AquI	6606	AquI	6885	AseI	646	AseI	3675	AseI	4910
AsnI	646	AsnI	3675	AsnI	4910	Asp26HI	489	Asp26HI	582	Asp26HI	6872	Asp27HI	489	Asp27HI	582
Asp27HI	6872	Asp35HI	489	Asp35HI	582	Asp35HI	6872	Asp36HI	489	Asp36HI	582	Asp36HI	6872	Asp40HI	489
Asp40HI	582	Asp40HI	6872	Asp50HI	489	Asp50HI	582	Asp50HI	6872	AvaI	6237	AvaI	6606	AvaI	6885
BcoI	6237	BcoI	6606	BcoI	6885	BfuAI	1759	BfuAI	2140	BfuAI	2592	Bli736I	3784	Bli736I	6066
Bli736I	6712	BluI	6237	BluI	6606	BluI	6885	BpoAI	646	BpoAI	3675	BpoAI	4910	BsaAI	992
BsaAI	2176	BsaAI	5421	BsaI	3784	BsaI	6066	BsaI	6712	BsaMI	489	BsaMI	582	BsaMI	6872
BscCI	489	BscCI	582	BscCI	6872	Bse15I	6237	Bse15I	6606	Bse15I	6885	Bse3DI	2105	Bse3DI	3616
Bse3DI	3790	BseMI	2105	BseMI	3616	BseMI	3790	BseRI	1674	BseRI	6216	BseRI	6794	BsgI	6128
BsgI	6501	BsgI	6778	BsiHKCI	6237	BsiHKCI	6606	BsiHKCI	6885	BsmBI	2813	BsmBI	2855	BsmBI	5670
BsmI	489	BsmI	582	BsmI	6872	Bso31I	3784	Bso31I	6066	Bso31I	6712	BsoBI	6237	BsoBI	6606
BsoBI	6885	BspAAI	6237	BspAAI	6606	BspAAI	6885	BspLU4I	6237	BspLU4I	6606	BspLU4I	6885	BspMI	1759
BspMI	2140	BspMI	2592	BspTNI	3784	BspTNI	6066	BspTNI	6712	BsrDI	2105	BsrDI	3616	BsrDI	3790
BssHI	6237	BssHI	6606	BssHI	6885	BstAPI	1423	BstAPI	1495	BstAPI	4921	BstBAI	992	BstBAI	2176
BstBAI	5421	BstENI	5926	BstENI	6480	BstENI	6715	BstGZ53I	2813	BstGZ53I	2855	BstGZ53I	5670	BstSI	6237
BstSI	6606	BstSI	6885	BstVI	6237	BstVI	6606	BstVI	6885	BveI	1759	BveI	2140	BveI	2592
CcrI	6237	CcrI	6606	CcrI	6885	Eco27kI	6237	Eco27kI	6606	Eco27kI	6885	Eco31I	3784	Eco31I	6066
Eco31I	6712	Eco88I	6237	Eco88I	6606	Eco88I	6885	EcoA4I	3784	EcoA4I	6066	EcoA4I	6712	EcoNI	5926
EcoNI	6480	EcoNI	6715	EcoO44I	3784	EcoO44I	6066	EcoO44I	6712	Esp3I	2813	Esp3I	2855	Esp3I	5670
MavI	6237	MavI	6606	MavI	6885	MlaAI	6237	MlaAI	6606	MlaAI	6885	MspYI	992	MspYI	2176
MspYI	5421	Mva1269I	489	Mva1269I	582	Mva1269I	6872	Nli3877I	6241	Nli3877I	6610	Nli3877I	6889	NspIII	6237
NspIII	6606	NspIII	6885	NspSAI	6237	NspSAI	6606	NspSAI	6885	OfoI	6237	OfoI	6606	OfoI	6885
PaeR7I	6237	PaeR7I	6606	PaeR7I	6885	PanI	6237	PanI	6606	PanI	6885	PasI	6278	PasI	6311
PasI	6747	PctI	489	PctI	582	PctI	6872	PlaAI	6237	PlaAI	6606	PlaAI	6885	PshBI	646
PshBI	3675	PshBI	4910	PsiI	70	PsiI	521	PsiI	1120	PsuAI	992	PsuAI	2176	PsuAI	5421
PunAI	6237	PunAI	6606	PunAI	6885	SapI	2216	SapI	2426	SapI	4855	Sau3239I	6237	Sau3239I	6606
Sau3239I	6885	SauLPII	6237	SauLPII	6606	SauLPII	6885	Sbi68I	6237	Sbi68I	6606	Sbi68I	6885	SciI	6239
SciI	6608	SciI	6887	Sfr274I	6237	Sfr274I	6606	Sfr274I	6885	SlaI	6237	SlaI	6606	SlaI	6885
SnoI	2681	SnoI	3178	SnoI	4424	Sol10179I	6237	Sol10179I	6606	Sol10179I	6885	Sru4DI	646	Sru4DI	3675
Sru4DI	4910	StrI	6237	StrI	6606	StrI	6885	TliI	6237	TliI	6606	TliI	6885	TspGWI	3069
TspGWI	3411	TspGWI	6836	VneI	2681	VneI	3178	VneI	4424	VpaK32I	2216	VpaK32I	2426	VpaK32I	4855
VspI	646	VspI	3675	VspI	4910	XagI	5926	XagI	6480	XagI	6715	XhoI	6237	XhoI	6606
XhoI	6885	XpaI	6237	XpaI	6606	XpaI	6885

Note: Position 1 is indicated above by the arrow pointing to the backbone start site.